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CB-839 was added to the final concentrations of 10 nM, 100nM, 1 M, 10 M, and 100 M in triplicate, and the cells were incubated for 72 hours

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Differential expression analysis The gene expression profiles derived from the TCGA and GTEx datasets were subjected to a log 2 (FPKM + 1) transformation to achieve normalization, thereby successfully mitigating batch effects