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Cultures were pulse-labeled with 10 M BrdU (a nucleoside analog that competes with thymidine for incorporation into DNA) in DMEM-10% HS for the final 2-h period of 48-h in culture, followed by immunocytochemistry for detection of BrdU using G3G4 anti-BrdU mAb (1:100 dilution) and HRP-conjugated anti-mouse IgG Ab (1:500 dilution)
The additional cost of adding TB-500 often proves worthwhile when faster, more complete healing matters
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Researchers interested in investigating epithalons safety profile should note that the peptide has not been formally reviewed by the United States Food and Drug Administration, nor by any of its international regulatory counterparts
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