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To create the tnaA mutant, the DNA fragment containing the kanamycin resistance cassette for homologous recombination was amplified by PCR using the primers F- tnaA -FRT and R- tnaA -FRT and the plasmid pKD4 as the template

Notably, cancer cells, with their elevated oxidative stress, exhibit heightened sensitivity to GSH deficiency, presenting a critical vulnerability exploitable in cancer therapy [11, 12]

Once protein is in place, adding vegetables, fiber-rich carbohydrates, and healthy fats becomes much easier

The Gila monster inspired drugs like tirzepatide as its venom contains hormones that can regulate blood sugar and impact appetite

Additionally, SAM plays a pivotal role as the primary provider of methyl groups, which are crucial for various processes, including gene regulation