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Intrinsic factor deficiency causes pernicious anemia, which may be associated with subacute combined degeneration of the spinal cord

Wash with PBS one more time and add 500 l of lysis buffer (0.1% Triton-X and 0.6% sulfosalicylic acid in 0.1 M potassium phosphate buffer with 5 mM EDTA at pH 7.5) on ice

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Its important to check their vitamin B12 levels often to adjust their treatment