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To assess if the metabolism of TNBS by NAPDH-linked enzymes in aerobic solution results in formation of ROS, we determined the formation of hydrogen peroxide in an aqueous solution (PBS) containing 0.25 mm NADPH, TNBS (1 mm) and GSSG reductase (15 U/ml) over time
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The assessment of free testosterone and DHEAS concentrations was performed by a commercial enzyme-linked immunosorbent assay) ELISA) kit (Abbott Healthcare Pvt
doi: 10.3390/ijms26031098 90 ShuWHYangSHWeiMLiuXCChenZXWeiCYet al
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