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Metabolomics analysis and data processing Metabolite extraction was performed by adding a cold solvent mixture of acetonitrile: isopropanol: water (3:3:2, v/v/v) to a homogenized tissue aliquot of 25 mg sea urchin gonad followed by centrifugation and drying of the supernatant as per Fiehn 37

For eliminating the potential baseline effect of different studies, the rates of body weight change from the initial values were selected as the therapeutic index

Cells were stained and analysed with a FACS analyser as stated above

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