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After 23 days following the sinking of all domes, 6 domes/line was embedded in Tissue Tek OCT compound (Sakura Finetek, #4583) and frozen in liquid N 2 using the SealN Freeze cryotray and box (Fisher Scientific, #NC1877501)
Early Cellular-Level Changes (Preclinical Findings) The earliest documented changes occur at the molecular level, within hours of receptor binding, and are not observable without laboratory measurement
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