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Time-lapse imaging Videos of freshly isolated mouse tubules stained with 50 nM SYTOX Green nucleic acid stain (Life Technologies) in the presence or absence of 150 nM Biotracker 609 Red Ca 2+ AM dye (Merck Millipore, 5.04297.0001) or 200 nM MitoTracker Red FM (Invitrogen, M22425) were obtained using an oil-immersion 63/0.3 EC Plan Neofluar objective
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The sound waves help disperse aggregates
Twenty-seven and 28 patients in the carnitine and control groups, respectively, received BCAA
For most adults, I suggest aiming for: 1.2-1.6 grams per kilogram of body weight for general health 1.6-2.2 grams per kilogram for those focused on body composition changes or very active individuals For a 150-pound person, this translates to roughly 80-120 grams of protein daily, spread across meals
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