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The cells were then washed with PBS, and GSH and GSSG levels were determined by applying the GSH/GSSG-Glo assay according to manufacturer instructions
Push up the plunger to expel almost all the air (fig
In this work, we show that ROXY9 has weak GSH-dependent de-glutathionylation activity on glyceraldehyde-3-phosphate dehydrogenase (GAPDH), but no oxidase or reductase activities on other substrates like HED, Cys-SG, cumene hydroperoxide (CHP) or roGFP2
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