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Following treatment, cells were washed twice with PBS and then treated with 10 mM 2,7-dichlorodihydrofluorescein diacetate (DCFH-DA) (Beyotime, Shanghai, China) at 37 C in the dark for 20 min
10.3389/fpsyt.2014.00179 6
Think of it as a repair dispatcher
The other drawback is that some people who are sensitive to higher histamine levels or who have mast cell activation syndrome report that NAC gives them a histamine reaction
This is the cardinal sin of peptide handling
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