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Electroporation buffer and unloaded RFP were removed by ultrafiltration via an Amicon centrifugal filter (Millipore, UFC510096, 100 kDa molecular weight cutoff) followed by three washes with DPBS buffer (10 mM, pH 7.4, 13,000 g) at 4 C
The reduced form (GSH) is the predominant one, accounting for more than 98% of total glutathione
Age-related Factors Age-related factors play a significant role in determining how long the effects of a B12 injection last
Side-effect peak at each dose increase, titrating down within 5-10 days at each stable step
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