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After 24 h, migrated cells on the upper surface of the filter were removed with a cotton swab, while the cells on the bottom of the filter were fixed with 4% paraformaldehyde at room temperature for 15 min and stained with 3% crystal violet solution for 15 min

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The cryo-EM maps and coordinates for the GluK2 in the intermediate state, deep-desensitized state 1, deep-desensitized state 2, and deep-desensitized state3 have been deposited in the EMDB under accession numbers EMD-48760, EMD-48759, EMD-48758, and EMD-48757, and in the PDB under accession codes, 9MZL, 9MZK, 9MZJ, and 9MZI, respectively

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