Real-time qPCR was performed using an Applied Biosystems QuantStudio 12 K Flex real time PCR system (Thermo Fisher Scientific, San Jose, CA, USA) with THUNDERBIRD SYBR qPCR Mix (Toyobo), 6 pmol of primers, and aliquots of cDNA
Use room temperature bacteriostatic water for reconstitution
Chewable antacids work more quickly than tablets, typically providing symptom improvement within 5-15 minutes
Lyophilized peptides are stable at ambient temperatures for several weeks, easily covering standard shipping times
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