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A broad panel of antibodies was employed, including those for FOXO4, p53, phospho-p53, p21, p16, cleaved-Caspase3, BAX, BCL-2, testosterone synthesis enzymes (3-HSD, CYP11A1, CYP17A1), and various SASP factors (IL-1, IL-6, TNF-, TGF-)

The concentration of complementary deoxyribonucleic acid (cDNA) after reverse transcription were also determined using NanoDrop Spectrophotometer

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It has been observed that the liver is used to store Vitamin B12 in excess to decrease the likelihood of deficiency

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