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For in vivo animal experiments, the pGV469-mito-CD147 scFv expression vector was packaged into adenovirus and injected intratumorally at multiple sites (10 9 pfu, 30 L) or intravenously into the tail of mice (10 8 pfu, 100 L)
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This setup gives researchers a practical way to study angiogenic upregulation, cytoskeletal dynamics, and extracellular matrix (ECM) remodelling all at once, rather than running separate isolated assays
Iron overload exacerbates insulin resistance and increases hepatic gluconeogenesis
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