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Resting microglia typically display long, ramified processes

The mechanism involves upregulation of tissue inhibitors of metalloproteinases (TIMPs) and simultaneous downregulation of matrix metalloproteinases (MMPs), shifting the extracellular matrix balance toward net deposition rather than degradation

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The 96-well assay plate media was then replaced with 180 l per well of freshly prepared assay media, and the plate was incubated at 37 C, no CO 2 , for 1 h to eliminate residues of carbonic acid

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