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The glutathione can then be degraded by the cytoplasmic ChaC family of -glutamylamine cyclotransferases [ChaC1 (ChaC glutathione specific gamma-glutamylcyclotransferase 1) and ChaC2 (ChaC glutathione specific gamma-glutamylcyclotransferase 2)] generating 5-oxoproline and the dipeptide Cys-Gly

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Thus, the residual absorbance only represents unreacted substrates, providing a contamination-free result

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