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Isotopically labeled internal standards included L-carnitine-(trimethyl-d9) (CAS 126827-79-0, Cambridge Isotope Laboratories, Tewksbury, MA, USA), trimethylamine-d9 hydrochloride (CAS 18856-86-5, LGC Standards, Teddington, UK), trimethylamine-d9 N-oxide (CAS 1161070-49-0, LGC Standards), and -butyrobetaine-d9 (CAS 479677-53-7, Santa Cruz Biotechnology, Dallas, TX, USA)

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