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334 Endothelial cells (ECs) covering the inner walls of blood vessels have recently been found to control osteogenesis

Complex was formed by adding 10 mM CaCl 2 , 10 mM MgCl 2 , 1 mM MnCl 2 , 50 mU/mL apyrase (Sigma-Aldrich, Darmstadt, Germany), 20 M GLP-2, 100 M TCEP (Sigma-Aldrich) and 10 g/mL Nb35 into the cell lysate and incubated at room temperature (RT) for 1.5 h

Distinct particles floating in the solution or settled at the bottom indicate either aggregation, precipitation, or contamination

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However, the impact of inhibiting ROCK was not as significant in vivo as in vitro [201] (Fig