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Each peptide class requires specific negative controls: Vehicle-only control Essential for every study to control for injection stress and handling Receptor antagonist control Recommended for mechanistic studies (e.g., exendin(939) for GLP-1 receptor studies) Positive control A known active comparator (e.g., metformin for metabolic studies, diazepam for anxiolytic studies) Common Pitfalls in Peptide Selection Overlooking species-specific pharmacology: GLP-1 receptor sequences differ between rodents and humans
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