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Reconstitution technique errors that destroy peptide integrity include: injecting bacteriostatic water directly onto the lyophilised powder (shear forces break peptide bonds), shaking the vial to mix (introduces air bubbles that denature surface peptides), and using non-sterile water or saline instead of bacteriostatic water (bacterial growth within 72 hours)

Cell lines offer the advantages of immortality and high reproducibility, facilitating long-term, continuous experimentation without needing to maintain low passage numbers

doi: 10.1016/j.cgh.2014.04.014 3

doi: 10.2147/CMAR.S196509 124 WangXZhouQYuZWuXChenXLiJet al

Similarly, no two-way interactions or main effects were observed in the AUC values for TG, TC, HDLc, or LDLc (Fig