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The present review summarizes the latest scientific studies that have investigated microorganism-derived riboflavin synthesis using different methods, such as media component optimization, mutations and screening, genetic engineering, and biocatalyst conversion, to improve the production of vitamin B2 and its precursors
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J Immunol (Baltimore Md: 1950) (2001) 167(8):468692
After fixing for 15-20 minutes, cells were washed in PBS, permeabilised in 0.25% Triton X-100, and blocked in 4% BSA