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In parallel, Per2-luciferase rhythms were monitored in synchronized cells cultured in DMEM containing 0.1 mM luciferin using a LumiCycle apparatus (Actimetrics)
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Gene silencing was verified by detecting proteins via immunoblotting analysis after transient transfection with siRNA
It's involved in many processes in the body, including tissue building and repair, making chemicals and proteins needed in the body, and immune system function
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