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The assay is based on the reaction of MDA with TBA under high temperature and acidic conditions to form a TBARS, producing a pink chromogen that was measured spectrophotometrically at 534 nm

The standard ratio is 2mL of bacteriostatic water per 5mg vial, producing a concentration of 2,500 mcg/mL

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Future studies should employ stratified analyses or doseresponse curves to explore the influence of different GSH doses on prognosis, offering guidance for more precise clinical application