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Key Takeaways MOTS-c is a peptide produced inside your mitochondria

Ten microliters of 0.1 mg mL 1 ShHTL7 protein in a reaction buffer containing 20 mM HEPES, pH 7.0, 150 mM NaCl and 80 L of reaction buffer were mixed in a 96-well black plate (Grenier)

GHRP-6s structural proximity to ghrelin makes it the preferred tool compound for probing these non-GH CNS functions Side-by-Side Research Comparison Selectivity Profiles in Depth GHSR-1a Binding Affinity All three bind GHSR-1a with high affinity but not identically, and binding affinity doesnt cleanly predict in vivo GH output

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Bhatt DL, Lincoff AM, Gibson CM, et al