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Faculty is clean and all staff has been pleasant to deal with
Quantitative real-time polymerase chain reaction (PCR) analysis Total RNA was extracted using a TRIzol kit (Invitrogen, Carlsbad, CA, USA) and cDNA synthesis was performed using a cDNA synthesis kit (Fermentas Life Sciences) according to the manufacturer instructions
It is an advanced procedure, given the proximity of the lungs and the potential complication of lung puncture
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