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Shi, M.M

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Kipnis (1964) showed that gastrointestinal factors were indeed responsible for the extra insulin secreted after oral glucose, and that this mechanism was markedly impaired in patients with type 2 diabetes ( [31] ) ( [27] )

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The derivatization protocol for the quantification of TMA was an adaptation of the protocol described by Samples for TMAO levels quantification were prepared as follows: an amount of 25 L of each sample was collected, and 3 L of D9-TMAO (5 M concentration) and 122 L of a cold acetonitrile:methanol mixture (50:50, v/v) were added for protein precipitation

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