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Required Equipment and Materials Plate reader (405 or 415 nm filter) 96-well microplate Incubator (37C) 20 l and 200 l pipettes, a multi channel pipette 5-Sulfosalicylic Acid (SSA) Solution Ethanol Assay Procedure Sulfasalazine Alters Intracellular Metabolites and Increases ROS in A549 Cells Induction of Ferroptosis by Erastin Erastin is a known inducer of ferroptosis

10.1016/j.bbagen.2014.12.001 Biochim Biophys Acta

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Since this is a multi-dose vial with a preservative, it can be used for multiple doses in the same patient