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Freezing the reconstituted solution Reconstituted Melanotan-1 is not frozen in standard practice freeze-thaw can damage the peptide
The authors thank the Center for Advanced Equipment and Educational Support, Faculty of Agriculture, Kyushu University for the use of the ECL-detection imaging system and the plate-reader
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The UHPLC system was operated at 400 L/min for 10 mins at 30 C using test mobile phase (Thermo Scientific)
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