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A modified two-dimensional quantification method was employed to count immuno-positive cells in the DCN 71,72
Yoo SE, Chen L, Na R, Liu Y, Rios C, Van Remmen H et al
Formation of hydrogen peroxide was determined after addition to the solution of 10 l of 2-7dichlorofluorescein diacetate (2m in ethanol) and DCF fluorescence measured as described previously (Garca-Ruiz et al, 1995, 1997)
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