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Most research-context protocols use 3.0 mL of bacteriostatic water for the 80 mg vial, which produces about 26.7 mg/mL total blend concentration
ME1 and ME3 require NADP + and ME2 utilizes either NAD + or NADP + for their catalytic activities, thus NADPH can be produced by ME both directly and indirectly through the activity of the NNT that catalyzes the transfer of hydride ions from NADH to NADP + and produces NADPH in mitochondria
This ratio reflects the redox state of the cell and is crucial in studies of cellular response to oxidative stress and toxicity
Malde, A
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