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At 7 dpi, the wells were fixed with 2 mL/well of 7% Formaldehyde (VWR,10790-708) for 30 min and with 0.5 mL/well of 1x crystal violet (VWR, 470337-534) for 15 min

The Glycemia Reduction Approaches in Diabetes: A Comparative Effectiveness Study (GRADE) was a multicentre open-label RCT designed to test four different diabetes medication classes in people with type 2 diabetes and compare their ability to achieve and maintain HbA 1c levels 40%), as demonstrated in the Empagliflozin Outcome Trial in Patients With Chronic Heart Failure and a Preserved Ejection Fraction (EMPEROR-Preserved) [151]