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Berelowitz M, Szabo M, Frohman LA, Firestone S, Chu L, Hintz RL
Transmission of -synuclein-containing erythrocyte-derived extracellular vesicles across the bloodbrain barrier via adsorptive mediated transcytosis: another mechanism for initiation and progression of Parkinson's disease
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Py230 cells (provided by Nicholas Webster and Lesley Ellies, University of California San Diego) were maintained in F12K Nutrient Mixture (Corning 10-025-CV) supplemented with 5% Fetal Clone II (Cytiva SH30066.03), 50 g/mL gentamycin (Sigma-Aldrich G1397), 2.5 g/mL amphotericin B (Sigma-Aldrich A2942), and 1 L/mL MITO+ Serum Extender (Corning 355006) at 37 C and 5% CO 2
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