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Deformability Optimization: Integrating biocompatible edge activators to lower the elastic modulus of the membrane, enabling the vesicles to physically squeeze through the stratum corneum via intercellular pathways

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For colony formation assays, cells (5 10 4 ) were suspended in 2 mL of 0.33% noble agar (Difco) and laid over 6-well culture plates previously coated with 2 mL of 0.5% noble agar