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Briefly, GBM cells (5 10 5 ) seeded in a 6-well plate were washed once with PBS and incubated in 2 ml of Krebs buffer (126 mM NaCl, 2.5 mM KCl, 25 mM NaHCO 3 , 1.2 mM NaH 2 PO 4 , 1.2 mM MgCl 2 , 2.5 mM CaCl 2 ) without glucose for 30 min at 37 C
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This involves performing blood tests to check their vitamin B12 levels and kidney and liver function, as well as watching out for any signs of allergies or unwanted side effects
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