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Luciferase reporter assays A bicistronic vector pcDNA3 RLuc POLIRES FLuc (empty reporter) (Addgene, 45642) and pcDNA3 RLuc POLIRES FLuc containing human PRKAA1 5UTR upstream of FLuc (5UTR reporter) were transfected into shCtrl and shGDH1 or shYBX1 Huh7 cells using Lipofectamine 2000 (Thermo Fisher)

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S8A), which also suggests that the effect of Sema on the heart may be independent of its effect on body weight

Psychiatry 179 , 611627 (2022)

Self-administration kits include syringes, Semoralin, Steri wipes and instructions on self administration