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Importantly, while recent genomic profiling indicates that some historically broad ovarian cancer cell lines (e.g., SKOV-3, A2780) may not strictly represent HGSOC, early foundational in vitro studiesparticularly those evaluating metastatic competenceheavily relied on these models

Supernatant containing the protein portion was added with sample buffer (0.125 M Tris [pH 6.8], 10% [v/v] glycerol, 0.02% SDS, and 1 mM dithiothreitol [DTT]) and subsequently heated at 95 C for 5 min

Speed of results Injectable GHK-CU tends to reach deeper tissue faster

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