One g of RNA was reverse transcribed into cDNA using the Transcriptor First Strand cDNA synthesis kit (Roche, Indianapolis, IN, USA) and subjected to quantitative PCR using the SYBR-Green Supermix (Roche, Indianapolis, IN, USA) with the ABI Stepone Plus real-time PCR detection system (Applied Biosystems, Foster City, CA, USA)
November 2023: Canadians filed a proposed class action lawsuit in B.C
If the reddening does not disappear after 24 hours do not use the product
Once reconstituted, stability drops to 28 days under optimal conditions
Some retention is expected, but dramatic puffiness or rapid weight gain from fluid warrants dose reduction