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It shows exactly how much solution must be drawn to reach a particular microgram dose, based on the concentration created during reconstitution

Hypoxic mice were administered hypoxic treatment in a closed hypoxic chamber (China Innovation Instrument Co., Ltd, Ningbo, Zhejiang, China), which accurately set the desired hypoxic concentration and pattern

Heliyon 6, e03803

After washing twice with UA as above, 100 L of 50 mM AmBic (50 mM NH 4 HCO 3 in water) was added to the filter unit and the filter was centrifuged at 14,000 g for 10 min

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