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Cysteine and glycine are generated from methionine and serine, respectively, and glutamic acid is synthesized from -ketoglutarate, a metabolite of glucose

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Once the catheter is in place, the needle comes out

As the experimental readout is image-based, we extracted multiple phenotypic features of infection, including parasite growth, progression of the parasite to its sexual life stage, and recruitment of host actin to pedestals beneath the parasite vacuole