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The reduced form of glutathione (GSH) is considered the essential non-enzymatic antioxidant in the body and the first line of defense against oxidants since it scavenges free radicals, undergoing oxidation to disulfide GSSG [154]
The reaction of superoxide with NO yields peroxynitrite, ONOO , a very potent oxidant that intensifies eNOS uncoupling by oxidizing its cofactor BH 4 [76]
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Bound proteins were eluted using the same buffer containing 50 mM reduced glutathione
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