The practice's direct-pay model ($125 annually for individual membership, $75 for follow-up visits) allows extended appointment times for comprehensive care and education
For mitochondrial ROS detection, the cells were stained with 5 g/mL MitoSox dye (Thermo Scientific, Waltham, MA, USA) at 37 C for 15 min, followed by three PBS washes
The RT protocol was based on a previously described procedure [17]
Naunyn-Schmiedeberg's Arch Pharmacol 399 , 1261312627 (2026)
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