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Fluorescence was determined at Reading1 (excitation 395 at nm, emission at 510 nm) and Reading2 (excitation at 475 nm, emission at 510 nm) by a microplate reader (BioTek Synergy H1, Agilent, United States)
NMN can enhance mitochondrial function by increasing NAD+ levels, which is crucial for the generation of ATP (adenosine triphosphate) through oxidative phosphorylation
It helps with signaling and cell growth
Total glucose and lactate concentrations from medium were determined using a YSI 2950D
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