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Cell viability assay Cellular viability of treated liver models was measured after 7 days of drug perfusion using the CellTiter-Glo Luminescent Cell Viability Assay (Promega, Madison, WI, USA)
The simpler and more commonly used research route
After blocking with 5% non-fat milk for 1 h at room temperature, membranes were probed with the following primary antibodies at 4 C overnight: anti-BRD4 (Abcam, ab128874, 1:1,000 dilution), anti-integrin v (Abcam, ab179475, 1:1,000 dilution), anti-integrin 3 (Abcam, ab119992, 1:1,000 dilution) or anti-tubulin (Abcam, ab6160 1:2,000 dilution)
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