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After reaching 70% confluence, fibroblasts were cultured for 24 h in 1% FBS DMEM and then incubated with either TGF1 10ng/ml (Peprotech, Dollard des Ormeaux, Quebec, Canada), GHK 10 nM (Sigma, Markham, Ontario, Canada) or control media (1% FBS DMEM, penicillin, streptavidin) for a further 48 h

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The RNA samples (1 ng) were reverse transcribed into cDNA using the Hiscript III Reverse Transcriptase Kit (Vazyme, R223-01)

In addition, pulling the code directly from EHR integration reduces manual transcription errors at submission