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In a typical experiment, 1020 g of total protein extract were loaded per lane in 2 Laemmli buffer containing bromophenol blue
Any one of those errors reduces peptide potency by 1030%
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This irritation is typically mild and temporary but can be uncomfortable
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More recent research suggests the story is more complicated
GCN5-like protein 1 (GCN5L1) controls mitochondrial content through coordinated regulation of mitochondrial biogenesis and mitophagy
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