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Subsequently, cells were blocked with blocking buffer (PBS, containing 3% BSA, 0.3% Triton X-100, and 10% goat serum

Simultaneously, even well-stored peptides undergo gradual hydrolysis, a chemical reaction with water that cleaves peptide bonds and creates inactive fragments

4.5.3.3 Current validation framework status Although numerous studies employ area under receiver operating characteristic curves exceeding 0.8 as diagnostic performance criteria, validation data from independent external cohorts remain insufficient

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And Im like 53 and a half, Clarkson replied