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P values were calculated using one-way ANOVA ( d, e, i, k, l )

Then, 1 l of the cleared reaction mixture was spotted on a thin-layer chromatography (TLC) plate, and the plate was subjected to TLC analysis using a solvent system containing n -butanol/isopropanol/formic acid/water in a ratio of 3:1:1:1 (by volume)

Before visiting the laboratory: For follow-up testing, using similar fasting conditions, collection timing, and laboratory methods may make trends easier to interpret