The supernatant was collected to quantify the amount of compound or substrate using LC-MS
doi:10.1016/j.actatropica.2020.105498 Sharifi F, Mohamadi N, Tavakoli Oliaee R, et al
Once NAC is absorbed, the body converts it into L-cysteine, which is then paired with glutamate and glycine
Potential influence of metal ions (Ca 2+ , Mg 2+ , Na + and K + ) on the fluorescence intensity of the nanosensor was also examined

References: Mercury and protein thiols: Stimulation of mitochondrial F1FO-ATPase and inhibition of respiration Thimerosal-Derived Ethylmercury Is a Mitochondrial Toxin in Human Astrocytes: Possible Role of Fenton Chemistry in the Oxidation and Breakage of mtDNA Mercury has an affinity for the brain References: The Toxicology of Mercury and Its Chemical Compounds (Clarkson, Thomas, Magos, Laszlo, 2006) (a rather complete introduction to mercury toxicity) "The concentration in brain is about 5 times than in scalp hair about 250 times the corresponding concentration in blood" "Animal data including primates indicate that brain levels in the newborn may be as high as five times the corresponding levels in the mother" "Experimental work on rats has shown that methylmercury gains entry into the endothelial cells of the bloodbrain barrier as a complex with cysteine (Kerper et al., 1992