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Mitochondrial membrane potential analysis The mitochondrial membrane potential was assessed by incubating oocytes after maturation for 30 min at 37C in 0.5 M JC-1 dye (5,5,6,6-tetrachloro-1,1,3,3-tetraethylbenzimidazolylcarbocyanine iodide
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How Much to Take and For How Long During a loading phase, youll take a high dose of creatine, typically between 20-25 grams per day